Transplantation of Dopaminergic Neurons in Combination with Mesencephalic Neural Stem Cells for Parkinson s Disease
Wang Mingguo
Li Zhigao
Yang Zhiyong
Wang Bo
Duan Kuijia
Zhang Hailong
Song Xiaobin
Deng Xingli
Abstract：Objective To explore the therapeutic effect of intracerebral transplantation of dopaminergic (DA) neurons in combination with ventral mesencephalic neural stem cells (mNSC) for Parkinson's disease (PD). Methods The rat embryonic mNSCs were isolated in vitro, and modified with enhanced green fluorescent protein (EGFP). The rat embryonic DA neurons were isolated, and labeled with CM-DiI. The classical 6-hydrodopamine damage models of PD rats were established. Then the cells were transplanted into the striatum of PD rats by stereotactic injection. The lateral rotations were induced by intraperitoneal injection of apomorphine (APO), and the improvements of movement disorders were evaluated. The survival, migration and differentiation of transplanted cells were identified by immunofluorescence histochemistry. Results The movement disorders were obviously improved by cell transplantation in PD rats, and the therapeutic efficacy of cotransplantation of DA neurons and mNSCs ( cotransplantation group ) was the most striking . With cotransplantation, more mNSCs were differentiated into DA neurons. Most transplanted cells stayed in the transplant areas, while a very few cells migrated to the surrounding areas. Conclusions The intracerebral transplantation of DA neurons in combination with mNSCs can considerably improve the movement disorders of PD rats. However, the molecular mechanism remains to be further studied.
Keywords：Parkinson diseaseneural stem cell transplantationdopaminergic neuronsrats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 127-130 )