Protective effects of edaravone on the retinal ganglion cells after cranio orbital injury in rats
Gao Chen
Zhu Wenxia
Li Zhiyun
Yao Pengfei
Jia Ping
Zhang Jingke
Abstract：Objective To investigate the mechanism of apoptosis in retinal ganglion cells (RGC) and protective effects of edaravone on rats after experimental cranio-orbital injury (COI). Methods A total of 144 rats (Sprague Dawley, clean grade) were randomly divided into control group (n = 48), injury group (n = 48) and treatment group (n = 48). The COI model was established and edaravone (30 mg/kg) was injected intraperitoneally to rats in treatment group for intervention therapy and no intervention was performed in rats in injury group. The content of reactive oxygen species (ROS) and apoptosis rate of RGC were detected 3, 6, 10, 14 d after COI. The morphology and ultrastructure of apoptosis RGC were also observed. Results The content of ROS in RGC in injury group was higher than that in treatment and control groups 6, 10 and 14 d after COI (P<0.05). The apoptosis rate of RGC in injury group was higher than that in treatment and control groups 3, 6, 10, 14 d after COI (P<0.05). The microstructure and ultrastructure of RGC showed the typical characteristics of apoptosis in injury group but appeared normal in treatment and control groups. Conclusions The content of ROS in RGC could increase significantly after COI, which results in apoptosis imbalance in RGC. Edaravone can prevent this pathological process by eliminating ROS, and thus having a protective effect on optic nerve and is worthy to be popularized clinically.
Keywords：cranio-orbital injuryedaravoneretinal ganglion cellsreactive oxygen speciesapoptosisrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 324-327 )