Effects of hypoxic preconditioning on hippocampal neurons and behavioristics in rats after traumatic brain injury
Zhuo Jianwei
Liu Jiachuan
Yang Yanyan
Zhang Yongming
Zhang Xing
Zhou Zhimin
Ma Tao
Shu Longfei
Abstract：Objective To explore th e effect of hypoxic preconditioning on hippocampal neuron activity in rats after traumatic brain injury and its mechanisms. Methods Seventy-two SD rats were randomly divided into control group (n=6), hypoxic preconditioning group (HPC, n=6), traumatic brain injury group (TBI, n=30) and traumatic brain injury after hypoxic preconditioning group (HPCT, n=30). TBI model was established by modified Freeny's method. The rats were put in hypobaric chamber for hypoxic preconditioning. The neurological functions of rats in 4 groups were evaluated by the Neurological Severity Score (NSS) and the expression of matrix metallproteinase-9 (MMP-9) and NeuN in hippocampus CA1 were observed by immunohistochemical staining 3, 12 h and 1, 7, 14 d after injury. Results Compared with control group, the expression of MMP-9 from 3 h to 7 d after injury in the TBI group was significantly increased, the NSS from 3 h to 14 d after injury was increased and the expression of NeuN was significantly decreased (P<0.05). Compared with control group, the expression of MMP-9 from 12 h to 7 d after injury in the HPCT group was significantly increased, the NSS from 3 h to 14 d after injury was increased and the expression of NeuN from 12 h to 14 d was significantly decreased (P< 0.05). Compared with TBI group, the expression of MMP-9 from 3 h to 7 d and the NSS from 12 h to 7 d was significantly decreased, while the expression of NeuN from 3 h to 7 d was increased significantly (P<0.05). Conclusions Hypoxic preconditioning can ameliorate the lesions of hippocampal neurons after TBI by reducing the expression of MMP-9 in the hippocampal region.
Keywords：craniocerebral traumahippocampushypoxic preconditioningmatrix metalloproteinase-9neural nucleirats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 320-323 )