Identification of the invasion related gene of non functioning pituitary adenomas by gene expression profiling
Feng Jie
Hong Lichuan
Li Chuzhong
Yu Shenyuan
Wu Yonggang
He Yue
Wang Hongyun
Li Dan
Wan Hong
Zhang Yazhuo
Abstract：Objective To explore the invasion-associated gene of non-functioning pituitary adenomas ( NFPA ) . Methods Gene expression microarray analysis was performed in 7 NFPA patients and 3 subjects with normal pituitary tissue. The invasion-associated genes of NFPA were selected through bioinformatics and statistical biological software. The correlation of ENC1 gene with aggressive NFPA was verified by QPCR and immunohistochemistry in 43 NFPA samples. Results A total of 56 genes were found to be related to the invasiveness in invasive NFPA, non-invasive NFPA and normal pituitary glands. Of them, expressions of 35 genes were up-regulated and those of 21 down-regulated in invasive NFPA compared with non-invasive NFPA and normal pituitary glands. The ENC1 expression level was significantly lower in invasive NFPA compared with non-invasive NFPA (P=0.010). Immunohistochemistry showed ENC1 was strongly positive in normal pituitary tissues and weakly positive in invasive NFPA. Conclusions ENC1 may be an important biomarker for invasive NFPA.
Keywords：pituitary neoplasmsnonfunctioninvasivenessgenearray
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 269-272 )