Preliminary study of miR 410 targeting on MET in gliomas
Zhang Junhe
Zhang Jun
Yang Dongbo
Li Yang
Ming Jianguang
Han Xuanye
Jiang Chuanlu
Abstract：Objective To explore the functional mechanism of miR-410 in glioma cells. Methods Fifty human glioma samples and glioblastoma cell lines U251 and U87 were collected. The genetic analysis of correlation of miR-410 and MET gene was judged by the miRanda target gene prediction software. Immunohistochemistry and in situ hybridization technique were used to confirm the expressions of miR-410 and MET in the human brain glioma samples. Further, Luciferase experimental method was used to test the targeting effect of miR-410 on MET. Western blot was used to detect the change of MET protein after the transfection of miR-410 analogue into U87 and U251 tumor cells. Results The matching sequences between 3'untranslated region (3'UTR) of MET mRNA and seed sequences of miR-410 existed theoretically, which were confirmed by miRanda target gene analysis. The negative correlation of miR-410 and MET in tumor samples was confirmed by immunohistochemistry and in situ hybridization (r=ˉ 0.69783, P<0.001). Luciferase experiments further confirmed that MET gene was the direct target of miR-410 in human glioblastoma. After the tumor cells were transfected with miR-410 simulation, the MET expression was inhibited significantly (P<0.05). Conclusion miR-410 targets on MET in gliomas, and may be the new target for glioma treatment.
Keywords：gliomamiR-410geneMETluciferase reporter assay
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 33-36 )