Construction of mouse intracranial infection model induced by S.aureus
Li Dongzhi
Yan Jun
Li Di
Liu Yu
Dong Jie
Gao Yaping
Yang Guang
Sun Tao
Gong Jian
Abstract：Objective To construct the mouse intracranial infection model induced by S.aureus. Methods One hundred and six female C57BL/6J mice (6 to 8 weeks old) were equally and randomly divided into experimental and control groups. S.aureus 8325-4 solution 5 μl (5×106 CFU) were injected into the brain of the mouse in experimental group, while sterile saline were injected into those of control group. After injection, clinical manifestations, the earliest time point of death and 48-h survival rate were recorded, rectal temperature and hemogram were monitored, and the brain tissues from the pathological section were observed for pathological study. Results After injection, the rats showed symptoms such as slow response and spasm, the earliest time point of death was 12 h, and the 48-h survival rate was 26.9% in the experimental group. Compared with control group, the percentage of neutrophil increased significantly, and the neutrophil granulocyte infiltration in the infection area of brain tissues was observed in experimental group. While there were no symptoms and dead rats in control group, and the brain tissue was normal based on pathological examination. Conclusion The mouse intracranial infection model induced by S.aureus is stable, and can be used in clinical study for pathogenic mechanismof intracranial infection induced by S.aureus.
Keywords：intracranial infectionstaphylococcus aureusmodelsanimal
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 315-318 )