Effect of hypoxic preconditioning on claudin 5 expression and blood brain barrier permeability after traumatic brain injury in rats
Yao Yinsheng
Liu Jiachuan
Yang Yanyan
Wang Jinbiao
Zhang Yongming
Zhang Xing
Wang Chunlin
Abstract：Objective To explore the effect of hypoxic preconditioning on tight junction protein claudin-5 and blood-brain barrier permeability after traumatic brain injury in rats. Methods 204 rats were randomly assigned into traumatic brain injury group (group T, n =96), traumatic brain injury with hypoxic preconditioning group (group H, n=96) and control group (n =12). The rats in group T were established traumatic brain injury model with freefall impact method, and the rats in group H were treated by the same methods after 3 days of hypoxic preconditioning, then the rats in groups T and H were decapitated at time point of 1 h, 4 h, 8 h, 12 h, 24 h, 3 d, 7 d, and 14 d after injury. The wet and dry weight method was used to detect the brain water content of rats. Real-time PCR and Western blotting were used to detect the changes of claudin-5 mRNA and protein in brain contusion area. IgG method was used to detect the changes of blood-brain barrier permeability. Results Claudin-5 mRNA and protein expressions of groups T and H began to decrease at 1 h, fell down to the bottom 8 to 12 h, began to rise gradually on 1 d, and tended to the level of control group until 14 d after injury. Additionally, claudin-5 mRNA and protein expressions in group H were higher than in group T at every time point. The blood-brain barrier permeability and brain water content at each time point in group T were significantly higher than those in group H (P<0.05), and those in the two groups were higher than those in control group (P < 0.05). Conclusion Hypoxic preconditioning can up-regulate claudin-5 expression in early traumatic brain injury, protect the integrity of blood-brain barrier and reduce cerebral edema.
Keywords：craniocerebral traumahypoxic preconditioningblood-brain barrierClaudin-5rats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 180-183 )