Establishment of middle cerebral artery occlusion model with modified intraluminal filament method and analysis of HSP70 expression in ischemic areas in rats
Li Hao
Wang Kai
Wang Tao
Abstract：Objective To establish the middle cerebral artery occlusion (MCAO) model and analyze the expression of HSP70 in the ischemic area in rats. Methods Sixty Wistar rats were randomly divided into MCAO group and sham operation group. MCAO model was established with modified intraluminal suture method, and the sham operation group received only ligation of right internal carotid artery. Hematoxylin and eosin staining was used to observe the morphological changes of the brain tissue in two groups, and immunohistochemical technique was used to detect expression of HSP70, and the statistical analysis was performed for the two groups. Results The MCAO model was established successfully in 23 rats and the success rate was 76.7%in MCAO group. Hematoxylin and eosin staining showed that the neurons suffered ischemic injury in MCAO group. The HSP70 expression was 7.32±0.894, 12.61 ±0.937 and 14.83 ± 1.395 in 6, 12, 24 h in MCAO group and 2.12 ± 0.751, 1.93 ± 1.237, 2.17 ± 0.352 in sham operation group respectively, and there was a statistically significant difference at every time point between the two groups (P<0.01). Conclusions Establishment of MCAO model with modified intraluminal thread method is simple, feasible, and of high success rate. HSP70 may increase the neuronal tolerance to ischemia and hypoxia, and resist the further lethal damage.
Keywords：brain ischemiainfarctionmiddle cerebral arteryheat-shock proteins 70intraluminal thread methodrats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 176-179 )