Thermal exchange efficacy of a novel blood perfusion device:in vitro and in vivo animal experimental study

Yu Xiang; Lin Jing; Hou Jianglong; Gu Jun; Tang Zongcheng; Wang Yishun; Du Lei; Xiong Jiyue

doi:10.13498/j.cnki.chin.j.ecc.2025.04.12

Abstract：Objective To evaluation of the thermal exchange efficacy of a novel blood perfusion device(XJ-40-20)for cardioplegia delivery.Methods According to the"Technical Requirements for Disposable Cardioplegia Perfusion Devices,"in vitro experiments were conducted comparing the novel XJ-40-20(experimental group,n=8)and a demestic comparator device(control group,n=8).Parameters measured included priming volume,cooling coefficients,and pressure drop.For in vivo evaluation,blood cardioplegia(blood:crystalloid ratio of 4:1)of 1250±0 ml was delivered by XJ-40-20 at a pressure of 149.7±6.4 mmHg for 5 minutes in a porcine cardiopulmonary bypass(CPB)model(n=6).In both in vitro and in vivo studies,the blood temperatures at the inlet and outlet of the perfusion device and the water temperatures were measured.In the animal study,the cardiac function was assessed after 90 minutes of aortic cross-clamping,and myocardial pathological examination was performed at 2 hours post-declamping.Results In vitro experiments although the prime volumes were similar between groups(P=0.30),the cooling coefficient of XJ-40-20 was significantly higher than that of the control group,with statistical significance.In animal experiments,XJ-40-20 effectively cooled the blood cardioplegia solution to 11℃ at a flow rate of 250 ml/min.Hearts were recovered after the 90-minute myocardial ischemia in all animals,with successful weaning from CPB after an average of 13.43±9.14 minutes of assistance.The normal structure of myocardial tissue was shown in pathological examination.Conclusion XJ-40-20 can provide satisfactory effects of cooling and myocardial protection for blood cardioplegia delivery.