Mechanisms of gut microbiota dysbiosis in exacerbating myocardial infarction via T cell immunity and inflammation and the effects of probiotic intervention
Wang Yali
Hao Shaoting
Liu Qinghua
Li Yuming
Yang Xianghong
Xia Yi
Yuan Xiaohe
Abstract：Objective To investigate the effects of intestinal flora disturbance in regulating T-cell immunity,cardiac inflammation and myocardial collagen deposition in mice with myocardial infarction(MI).Methods A mouse model was established and the mice were divided into four groups:Sham,MI,MI+antibiotic treatment(MI+ABX)and MI+Probiotics intervention.Cardiac function was detected by ultrasound,and the changes of intestinal flora in mice in each group were analyzed.The spleen and heart Treg cells,as well as IL-17,IL-23 and IFN-γ,as well as myocardial collagen fiber deposition were detected by ELISA.Results Compared with Sham group,there were significant changes in the proportion of intestinal flora in all groups.Echocardiography revealed impaired cardiac function in the MI and MI+ABX groups,whereas probiotic intervention partially preserved cardiac function in the MI+Probiotics group.ELISA demonstrated elevated Treg levels in the MI group and decreased in the MI+ABX group,and restoration of Treg populations following probiotic treatment.In addition,the expression of cardiac inflammatory markers and myocardial collagen deposition was up-regulated in both the MI and MI+ABX groups,while the increase of inflammatory markers and collagen deposition was reversed to some extent in the MI+Probiotics group.Conclusion The imbalance of intestinal flora may aggravate the pathological process of myocardial infarction by affecting T cell immunity,cardiac inflammation and myocardial collagen fiber deposition.Probiotic intervention can significantly improve intestinal flora imbalance,restore heart function,reduce inflammatory response and myocardial fibrosis.
Keywords：Intestinal floraMyocardial infarctionT-cell immunityHeart inflammationCollagen depositionVentricular remodeling
Publication Date：2025-06-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 263-268 )