Study on the protective effect of citraconate on myocardial ischemia-reperfusion injury in mice
Feng Linqi
Chen Cheng
Shan Wenju
Jian Yuhong
Yi Wei
Abstract：Objective To investigate the role of citraconate in LPS/IFN-γ induced inflammation of RAW264.7 macrophages and its effect on myocardial ischemia-reperfusion injury(MI/RI)in mice.Methods The inflammatory response of RAW264.7 macrophages was induced by LPS/IFN-γ,and the cell viability after citraconate treatment was evaluated by cell counting reagent(CCK-8).The inflammatory response of RAW264.7 macrophages was evaluated by real-time fluorescence quantitative PCR and western blot.The MI/RI model was constructed to compare the changes in cardiac function after intraperitoneal injection of exogenous citraconate in mice.The inflammatory response of the heart after MI/RI was determined by fluorescence immunostaining,real-time fluorescence quantitative PCR,and protein immunoblotting.The expression of BAX(Bcl-2 associated X)protein and apoptosis of myocardial cells after MI/RI were measured by Tunel.Results The dosage and treatment time of citraconate for LPS/IFN-γ-induced RAW264.7 macrophages were determined to be 1 mmol/L to 10 mmol/L for 4 hours;citraconate significantly alleviated LPS/IFN-γ induced inflammation in RAW264.7 macrophages and exhibited a dose-dependent effect.Citraconate treatment significantly reduced cardiac inflammation and myocardial cell apoptosis in mice after MI/RI,and significantly improved cardiac function in mice after MI/RI.Conclusion Citraconate improves the cardiac function of mice after MI/RI by alleviating cardiac inflammation and myocardial cell apoptosis.
Keywords：CitraconateMyocardial ischemia-reperfusion injuryRAW 264.7 macrophagesCardiac functionInflammationMyocardial cell apoptosis
Publication Date：2025-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 162-169 )