Platelet count and function during extracorporeal membrane oxygenation in sheep model

Yan Shujie; Wang Tianlong; Wang Jing; Zhang Han; Lou Song; Teng Yuan; Wang Jian; Liu Gang; Ji Bingyang

doi:10.13498/j.cnki.chin.j.ecc.2025.01.16

Abstract：Objective This study aimed to investigate the effects of extracorporeal membrane oxygenation(ECMO)on platelet count and function in a sheep model of long-term awake veno-venous(V-V)ECMO and veno-arterial(V-A)ECMO.Methods Ten adult male small-tailed Han sheep were included in this study.Five were cannulated with dual-lumen catheters in the right jugular vein for V-V ECMO,and the other five were cannulated via the right jugular vein and right common carotid artery for V-A ECMO.After running ECMO for 168 hours,it was withdrawn.Platelet counts,ADP-induced platelet aggregation(ADP-PAG),arachidonic acid-induced platelet aggregation rate(AA-PAG),and thromboelastography maximum amplitude(TEG-MA)were tested before and during ECMO at different time points.A repeated measures ANOVA was used to compare the differences in platelet counts,functional parameters at different time points and ECMO modes.Results All 10 experimental sheep survived 7 days on ECMO.The main effects of time and ECMO mode on platelet counts(P=0.015)and TEG-MA(P=0.025)were statistically significant.The platelet count and TEG-MA were lower at 24 hours than those at 0 hours,and gradually increased later on.The main effects of time on ADP-PAG(P=0.318)and AA-PAG(P=0.157)were not statistically significant.The main effects of different ECMO mode on all platelet counts and functional indicators were not statistically significant(P values were 0.246,0.093,0.347).Conclusion ECMO caused a reduction in platelet counts and a marked decrease in the clot firmness within 24 hours,and recovered after 96 hours of ECMO support.