Study on the role of ophiopogonin D in pressure overload-induced myocardial hypertrophy in mice

Su Zheng; Dong Ke; Ding Wenyuan; Zhai Mengen; Su Jie; Zuo Qian; Liu Jincheng; Gu Chunhu; Han Yuehu; Wang Hui

doi:10.13498/j.cnki.chin.j.ecc.2024.06.14

Abstract：Objective To investigate the protective effect of ophiopogonin D (OP-D) on pathological myocardial hypertrophy induced by pressure overload in mice. Methods Transverse aortic constriction (TAC) was established to induce pressure overload pathological myocardial hypertrophy in mice. C57/BL6J mice were randomly divided into four groups using a random number generator,with eight mice in each group:Sham+Vehicle group,Sham+OP-D group,TAC+Vehicle group and TAC+OP-D. OP-D was administered via intraperitoneal injection. Cardiac function was evaluated by detecting left ventricular ejection fraction (LVEF) and short axis shortening rate (FS) using echocardiography. Pathological hypertrophy was assessed by measuring interventricular septum thickness (IVSd) and left ventricular posterior wall thickness (LPWd) of mice were measured by echocardiography,heart weight was weighed,the ratio of mouse heart weight to tibial length ratio (HW/TL),and calculating the average cardiomyocyte cross-sectional area (CSA) by wheat germ agglutinin staining. Cardiac fibrosis was detected by Masson staining. qPCR was used to detect the transcription levels of hypertrophy related molecules Nppa,Nppb,Myh7,and fibrosis related molecules Tgfb1,Col1a1,and Col1a3. Results Compared with the TAC+Vehicle group,after OP-D treatment,LVEF and FS in the TAC+OP-D group were significantly increased (P<0.01 and P<0.01);IVSd,LVPWd,heart weight,HW/TL,and the average CSA of myocardial cells were significantly reduced (P<0.01,P<0.05,P<0.01,P<0.01,and P<0.05,respectively);The fibrosis index significantly decreased (P<0.001);the transcription levels of Nppa,Nppb,Myh7,Tgfb1,Col1a1,and Col1a3 in cardiac tissue were all significantly increased (P<0.05,P<0.001,P<0.05,P<0.001,P<0.001,and P<0.01). Conclusion OP-D improved cardiac function,reduced myocardial hypertrophy,and alleviated fibrosis in TAC mice.