Effect of Buyang Huanwu Decoction pretreatment on myocardial necrotic apoptosis after myocardial ischemia/reperfusion injury in rats

Zhang Guoxin; Ren Wenpu; Cao Zijun; Meng Shan; Wu Songzhe; Yu Liming; Zhu Yan; Zhao Jikai

doi:10.13498/j.cnki.chin.j.ecc.2024.02.14

Abstract：Objective To explore the effects of Buyang Huanwu Decoction pretreatment on the expression of protein receptor-interacting protein kinase 1(RIP1),receptor-interacting protein kinase 3(RIP3)and mixed series protein kinase-like structure(MLKL)in myocardial ischemia/reperfusion injury(MI/RI)rats.To explore the mechanism of regulation of necrotic apoptosis by Buyang Huanwu Decoction pretreatment during MI/RI process.Methods 40 SD rats were randomly divided into sham operation group,model group,low-dose Buyang Huanwu Decoction group and high-dose Buyang Huanwu Decoction group,with 10 rats in each group.Rat MI/RI model was established by ligation of the left anterior descending coronary artery(LAD).Buyang Huanwu decoction low-dose group and Buyang Huanwu Decoction high-dose group were given different concentrations of Buyang Huanwu Decoction by intragastric administration before modeling,sham operation group and model group were given normal saline intragastric administration.ECG monitoring was used to determine whether the modeling was successful,echocardiography was used to examine the differences in postoperative cardiac function of the rats,enzyme-linked immunosorbent assay(ELISA)was used to detect the activity of serum lactate dehydrogenase(LDH),hematoxylin-eosin staining and in situ end labeling were used to detect myocardial cell injury and apoptosis.The expression levels of RIP1,RIP3 and MLKL in rat heart tissue were detected by western blot.Results Compared with the sham operation group,the cardiac function of the model group was significantly decreased,the inflammatory infiltration of cardiomyocytes was obvious,and the apoptosis rate of cardiomyocytes,the expression of RIP1,RIP3 and MLKL necrotic apoptosis-related proteins were significantly increased.Compared with the model group,the pretreatment of Buyang Huanwu Decoction could improve the cardiac function of rats,improve the inflammatory infiltration of cardiomyocytes,and significantly reduce the apoptosis rate of cardiomyocytes,the expression of RIP1,RIP3 and MLKL necrotic apoptosation-related proteins.In addition,the improvement effect of Buyang Huanwu Decoction high dose group was more significant than that of low dose group.Conclusion Buyang Huanwu Decoction can reduce serum LDH activity,inhibit inflammatory response and improve cardiac function in MI/RI rats,the mechanism of which may be related to inhibiting the necrotic apoptosis of cardiomyocytes after reperfusion.