Bergenia alleviates simulate ischemia/reperfusion injury of cardiomyocytes in rats via SIRT1/PCG-1 α

Du Yanmei; Tian Zhansong; Tan Yanzhen; Sun Yang

doi:10.13498/j.cnki.chin.j.ecc.2024.01.10

Abstract：Objective To explore the molecular mechanism of Bergenin in alleviating ischemia-reperfusion(SI/R)injury in rat cardiomyocytes(H9C2).Methods H9C2 cells were modeled to simulate SI/R damage,the expression of key molecules was detected by WB,cell damage was detected by TUNEL staining and LDH release,intracellular reactive oxygen species levels were detected by DCFH-DA staining and MDA,and SIRT1 and PGC-1α were inhibited by EX-527 and SR-18292,respectively.The experimental groups were:the intervention group(SI/R+Ber),the intervention group(SI/R+Ber+EX-527),and the control group(SI/R+PBS).Results Compared with the SI/R+PBS group,the expression level of Bcl-2 in the SI/R+Ber group increased significantly(P<0.001),the expression level of Bax decreased significantly(P<0.001),and the expression level of PGC-1α increased significantly(P<0.001).Compared with the SI/R+Ber group,the expression level of Bcl-2 in the SI/R+Ber+Ex-527 group decreased significantly(P<0.001),the expression level of Bax increased significantly(P<0.001),and the expression level of PGC-1α decreased significantly(P<0.001).After administration of the inhibitor SR-18292 of PGC-1α,cell viability was significantly reduced compared with the SI/R+Ber group(P<0.01),LDH release was significantly increased(P<0.001),MDA production was significantly increased(P<0.01),apoptosis index of cardiomyocytes was significantly increased(P<0.01),ROS production was significantly increased(P<0.01),Bcl-2 expression level was significantly decreased(P<0.001),Bax expression level was increased significantly(P<0.01),while SIRT1 expression level was not significantly changed(P>0.05).Conclusion Bergenin activates SIRT1/PGC-1 α pathway to reduce ROS production,and then inhibit myocardial cell apoptosis in myocardial SI/R injury.