Protective effects of tetrahydrocurcum on myocardial ischemia reperfusion injury of H9c2 cells
Feng Jianmei
Feng Jianyu
Wu Yan
Zhai Mengen
Feng Xiao
Jin Zhenxiao
Cao Lei
Abstract：Objective To investigate the protective effects and underlying mechanisms of tetrahydrocurcumin (THC) pretreatment on ischemia reperfusion injury (IRI) of H9c2 cells. Methods To mimic the in vivo ischemia reperfusion model, H9c2 cells were exposed to an ischemic buffer for 1 h followed by serum-free DMEM for 4 h. Prior to this procedure, cells were treated with or without THC. They were divided into four groups: Control group, THC group, SIR group and SIR+THC group, respectively. Four hours after the reperfusion, cell viability, reactive oxygen species (ROS) production, malonaldehyde (MDA) content, activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as the expression levels of Bax, Bcl-2 and Caspase-3 were measured in each group. Results Compared with SIR group, THC pretreatment increased the cell viability, the expression of Bcl-2 and the activities of SOD and GSH-Px in H9c2 cells. In addition, THC also significantly decreased the apoptotic ratio, the expression of Bax and Caspase-3, ROS production and MDA content. Conclusion THC pretreatment could effectively attenuate myocardial IRI by reducing SIR-induced oxidative stress and apoptosis.
Keywords：TetrahydrocurcuminCellMyocardial ischemia reperfusion injuryApoptosisOxidative stressPretreatment
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 48-53 )