Compound APG derived from Clematis tangutica ameliorates myocardial ischemia/reperfusion injury via activating PKCε and inhibiting apoptosis
Feng Ying-da
Feng Jian-yu
Yang Yang
Di Shou-yin
Zhang Wei
Lu Yun-yang
Tang Hai-feng
Zhu Yan-rong
Abstract：Objective To investigate the protective effects of APG on myocardial ischemia/reperfusion (I/R) injury (IRI) and its possible mechanism.Methods The cultured H9C2 cells were treated with APG for 24 h,and subjected to IRI (I 45 min,R 3 h).After the 3 h reperfusion,cell viability,serum LDH and serum MDA,cell SOD activity,apoptotic index,PKCε expression,Caspase-3 expression,Bax expression and Bcl-2 expression were detected,then the specific inhibitor of PKCε CHE was applied to evaluate the roles of PKCε in this process.Results I/R treatment reduced cell viability significantly,decreased SOD activity and the Bcl-2/Bax ratio,down-regulated PKCε expression,up-regulated LDH levels,MDA levels and Caspase-3 expression (vs the control group,P<0.05).Moreover,APG treatment (both 2 μM/L and 4 μM/L) increased the cell viability,SOD activity and the Bcl-2/Bax ratio,down-regulated LDH levels,MDA levels and Caspase-3 expression,up-regulated PKCε expression in a dose dependent manner (vs the I/R group,P<0.05).In addition,compared with the APG+IR group,CHE treatment reduced the cell viability,up-regulated Caspase-3 expression,decreased the Bcl-2/Bax ratio (vs the APG+I/R group,P<0.05).Conclusion APG treatment ameliorates I/R injury via activating PKCε pathway and inhibiting apoptosis.
Keywords：Clematis tanguticaMyocardial ischemia/reperfusion injuryPKCεApoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 42-46 )