Comparisons between novel minimally invasive and classic transverse aortic con-striction surgery model in mice
Zhai Meng-en
Li Bu-ying
Duan Wei-xun
Yang Yang
Luo Li-yang
Yu Li-ming
Liu Bao-hui
Liang Hong-liang
Jin Zhen-xiao
Yu Shi-qiang
Abstract：Objective To comprehensively and technically introduce a novel transverse aortic constriction( TAC) surgery mod?el in mice and compare this relatively less invasive surgical modelto the classic TAC model. Methods C57BL/6 mice were divided in?to four groups:Sham-Classic;TAC-Classic;Sham-Novel;TAC-Novel. Mean operating time and overall survival rate during operating time and at week-4 were measured. Echocardiography was used to determine cardiac function. HW/BW, HW/TL and HE staining were used to measure the degree of cardiac hypertrophy. Results The average operating time using classic TAC surgery is 24.88±2.99 min, while TAC-N requires only 6.90±0.63 min ( P<0.01). The survival rate is 80% (TAC-C) vs 93.3% (TAC-N). No significant difference was shown in echocardiograph, HE staining, HW/BW and HW/TL between these two models. Conclusion This new ap?proach is more efficient and safer for mice while not compromising the extent of cardiac hypertrophy.
Keywords：Cardiac hypertrophyTransverse aortic constrictionThoracotomyMinimally invasive surgery
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 174-177 )