In vitro coagulation studies of hemodilution with different priming solution
Bian Lu-yu
Zhou Yu-jiao
Gao Guo-dong
Hei Fei-long
Liu Jin-ping
Long Cun
Yu Kun
Abstract：[ Abstract]:Objective To compare the effects of in vitro hemodilution with lactated Ringer's solution, hydroxyethyl starch 130/0.4 and 4% succinyl gelatin on the blood coagulation function. Methods Blood was taken from 10 healthy young male volunteers. Blood was diluted by 10%, 30%, and 50% using either 6% hydroxyethyl starch 130/0.4 ( Group W) , 4% succinyl gelatin ( Group G) and Ringer's lactate solution ( Group L) . Thrombelastograph ( TEG) was performed and record reaction time ( R time) , blood clotting time ( K time) , alpha angle, maximum amplitude ( MA) , at the same time conventional coagulation parameters were detected, such as hemoglobin( Hb) , prothrombin time ( PT) , prothrombin time activity ( PTA) , international normalized ratio ( INR) , partial thrombo?plastin time ( APTT) , thrombin time ( TT) and fibrinogen ( FIB) . Results R time was shortened and MA was increased in L group at 10% dilution ( P<0.05) . Negative effects on the coagulation system were shown in the Group G and Group W at 30% dilution ( P<0.05) , but most coagulation parameters were in the normal range. The coagulation function were impaired in all group at 50% dilution ( P<0.05) . Conclusion Coagulation function was closely related to the degrees of dilution. At 10% dilution, the three fluids do not significantly affect hemostasis.At 30% dilution, hydroxyethyl starch 130/0.4 and gelatin impaired the coagulation system, but the whole coagulation function was intact. At 50% dilution, the hemostasis was impaired greatly in all groups.
Keywords：Extracorporeal circulationHemodilutionCoagulation function
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6-10,54 )