Brain protective effect of antegrade selective cerebral perfusion with sodium pyruvate in deep hypothermic circulatory arrest
Wang Tian
Wu Shu-bin
Zou Li-hua
Liu Jin-ping
Ji Bing-yang
Tang Yue
Wu Ai-li
Abstract：[ Abstract]:Objective To explore whether a combination of sodium pyruvate ( Pyr) and blood for antegrade selective cerebral perfusion (ASCP) during deep hypothermic circulatory arrest (DHCA) could attenuate oxidative stress and exert a better protective effect on the brain. Methods Fourty-five rabbits were selected randomly. Eighteen were used for blood donation, other rabbits used for model establishment and which were divided into:Sham group (n=9), ASCP group (n=9) and Pyr group (n=9). The Sham group underwent anesthesia and thoracotomy,set up cardiopulmonary bypass ( CPB) but no on pump. During DHCA, ASCP was per?formed with a combination of 154 mmol/L isotonic solution of sodium pyruvate and blood for Pyr group but a combination of 154 mmol/L Nacl solution and blood for ASCP group. Blood gas and hemodynamic parameters were measured in five time points during CPB. The rabbits ended with euthanasia after CPB, and their brain tissue were preserved. All samples were used to measure malondialdehyde ( MDA) content, superoxide dismutase ( SOD) activity and S100B content. Results he MDA content of ASCP group was higher than Sham group and Pyr group ( P<0.05) . The SOD activity of ASCP group was lower than Sham group and Pyr group ( P<0.05) . The S100B concentrations of ASCP group and Pyr group at T3, T4 and T5 points were higher than T1 point in the same groups ( P<0.05) . The S100B concentrations of ASCP group at T3, T4 and T5 points were higher than Sham group and Pyr group at the same points ( P<0.05) . Conclusion The combination of blood and sodium pyruvate for ASCP during DHCA could exert a better protective effect on the brain by attenuating oxidative stress.
Keywords：Deep hypothermic circulatory arrestAntegrade selective cerebral perfusionAnimal modelSodium pyruvate
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 245-249 )