Research progress on PNH clone screening and diagnosis
LI Li-yan
FU Rong
Abstract：Paroxysmal nocturnal hemoglobinuria(PNH)is a rare acquired clonal disease of hematopoietic stem cells,clinically characterized by intravascular hemolysis,bone marrow failure,and high-risk concurrent thrombosis.Although it is a benign intravascular hemolytic disease,severe cases can be life-threatening.PNH has diverse clinical manifestations,complex symptoms,and is prone to multiple complications,often resulting in delayed diagnosis,misdiagnosis,and missed diagnosis.The classic complement hemolysis test lacks sensitivity and specificity for the diagnosis of PNH.The application of high-sensitivity flow cytometry has made breakthrough progress in the screening and diagnosis of PNH clones.The article provides a review of the current status and prospects of PNH,clone screening,and clinical application of flow cytometry.
Keywords：paroxysmal nocturnal hemoglobinuriaclone screeningdiagnosisflow cytometry
Publication Date：2025-07-01
Online Publishing Date：2025-09-04(First online date of this platform, not the publication date of the document)
Pages：8( 541-547,553 )