Value of determining Th17 and regulatory T cells in the peripheral blood in evaluating patients with systemic lupus erythematosus.

HAO Hui-qin; ZHANG Shao-ran; LI Xiao-feng; CHEN Jun-wei; LUO Jing; LYU Zhi-qin

doi:10.19538/j.nk2018030116

Abstract：Objective To study the value of determining Th17 and regulatory T cell(Treg)in the peripheral blood in evaluating patients with systemic lupus erythematosus(SLE). Methods This study included 100 patients with SLE (37 patients were in inactive SLE group and 63 patients were in active group) and 30 normal controls. Percentage of Th17 and Treg in the peripheral blood were detected by flow cytometry. The concentration of interleukin-17 was detected by cytometric bead array(CBA). The concentration of transforming growth factor-β was detected by ELISA. Results (1)The percentage of Th17 in the peripheral blood in patients with SLE was higher than that in normal controls (P<0. 05), and the percentage of Th17 in active SLE patients was higher than that in inactive SLE patients (P<0. 05). (2)The percentage of Treg in patients with SLE was lower than that in normal controls(P<0. 05), and the percentage of Treg in active SLE was lower than that in inactive SLE patients(P<0. 05). (3)The percentage of Th17/Treg level in active SLE patients was higher than that in inactive SLE patients and normal controls(P<0. 05). (4)The concentration of interleukin-17 in patients with SLE was higher than that in normal controls (P<0. 05), and the concentration of interleukin-17 in active SLE patients was higher than that in inactive SLE patients and normal controls(P<0. 05). The concentration of TGF-β had no difference between SLE patients and normal controls(P>0. 05), and it had no difference between active SLE patients and inactive SLE patients(P>0. 05). Conclution Th17 and Treg contribute to the pathogenesis of SLE, and Th17/Treg level can be the reliable index of SLE condition evaluation.