Applications of the detection of GAD65 reactive T cells in latent autoimmune diabetes in adult
WANG Wan-ru
YU Jing
SUN Zhi-chun
WANG Ai-hong
HONG Zhen-zhen
ZHANG Yi
ZHOU Zhi-guang
Abstract：Objective To explore the application of GAD65 reactive T cells(GAD65-T) detected by enzyme linked immune spot (ELISPOT) in screening LADA patients.Methods Totally 28 LADA patients,40 T2DM(Type 2 diabetes mellitus) patients and 20 healthy controls were recruited.GAD65-Ab were determined by radioligand assays.Human peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradients.ELISPOT assay was performed to measure the number of spots-forming plaque.One spot-forming represents a GAD65-T cells.The clinical characteristics of two groups(ELISPOT positive or negative) were compared by ELISPOT in 40 T2DM patients.Results Compared with T2DM and control group,the number of GAD65-T cell significantly increased in LADA group (P＜0.01),there being significantly more GAD65-T cells in T2DM than in control group(P＜0.05).A positive response was defined when the cell number ＞ 95％CI of control subjects.The positive rate was 71.4％ (20 of 28) in LADA group,37.5(14 of 40) in T2DM group and 0 in control group.In 40 T2DM patients,the patients in ELISPOT-positive subgroup had lower BMI,higher HbA1c and worse β cells function compared with the negative subgroup.Conclusions GAD65-T cells are found in some patients initially diagnosed with T2DM(GAD65-Ab negative),combination of enzyme linked immune spot and GAD65-Ab assay can improve the detection rate of LADA.
Keywords：diabetesautoimmunelatentglutamate decarboxylaseT-lymphocytesenzyme linked immune spot assay
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 617-620 )