Protein fingerprinting for screening specific biomarkers in serum of patients with ankylosing spondylitis
MA Dan
ZHANG Li-yun
ZHANG Gai-lian
XU Ke
HOU Rui-hong
GAO Jin-fang
Abstract：Objective The surface-enhanced laser desorption ionization/time of flight mass spectrometry (SELDI-TOF-MS) and protein chip was used for screening specific biomarkers in serum of patients with ankylosing spondylitis (AS) to diagnose and assess the disease as well as to anticipate the progress of disease.Methods The serum samples of 69 AS patients,10 patients with rheumatoid arthritis and 12 healthy individuals were detected by SELDI-TOF-MS and weak cation exchange chip.Then 69 AS patients were divided into several groups such as the active and inactive stage of illness,axial arthritis involved and peripheral and axial arthritis involved,and the positive and negative group of HLA-B27,to study proteins differentially expressed in the pathogenesis of AS.By using Biomarker Wizard and Biomarker Pattern software of SELDI to screen the specific proteins,set up the diagnostic prediction models of disease.Results The first diagnostic model made up of 8085,2640 and 2932 could be used to diagnose AS,whose the sensitivity and specificity were 94.23％ and 100％ respectively.The second diagnostic model made up of 3677,3880,2539,3159 and 3242 could be used to determine the disease activity of AS,whose the sensitivity and specificity were 98.11％ and 100％ respectively.The third diagnostic model made up of 4700,8687 and 18538 could be used to predict AS,whether peripheral arthritis be involved or not,whose the sensitivity and specificity were 80.00％ and 82.35％ respectively.The fourth diagnostic model made up of 10259,7972,2048,2154 and 2954 could be used to distinguish AS from RA,whose the sensitivity and specificity were 100％ and 100％ respectively.Conclusion The serum protein fingerprinting set up by SELDI-TOF-MS can screen specific protein biomarkers in AS,which is expected to become a screening platform in diagnosis and assessment of disease.
Keywords：ankylosing spondylitisprotein fingerprintingsurface-enhanced laser desorption ionization / time of flight mass spectrometryprotein chipserum
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 233-237 )