Application of soluble B7-H2 detection in early evaluation and its clinical significance on severity of patients with acute pancreatitis

HUANG Jian; ZHANG Guang-bo; HE Guang-sheng; SHEN Jia-qing; KONG Xiao-ming; SHEN Yue-ping

doi:10.7504/nk2015020402

Abstract：Objective To explore the clinical value of soluble B7 Homolog 2 (sB7-H2) on severity of patients with acute pancreatitis.Methods A total of 75 patients with AP were recruited to the study,along with 20 healthy volunteer controls (HC).Peripheral blood from patients with AP was collected at the first 24 hours after the onset of abdominal pain.The sB7-H2 levels were measured by ELISA.T-he association between sB7-H2 and clinical parameters were analyzed.Additionally,the sensitivity and specificity were examined through Receiver operating characteristic (ROC) analysis.Results The plasma level of sB7-H2 at 24h of the patients in AP group was significantly higher than that in healthy control group (P ＜0.01),It was no markedly different between in group of MAP and healthy control group(P＞0.05); The Plasma level of sB7-H2 inMSAP and SAP group were significantly higher than that inHC group(P＜0.05 and P＜0.001)and MAP group(P＜0.05 and P＜0.01); The Plasma level of sB7-H2 in SAP group was also obviously higher than that of MSAP group (P ＜ 0.05).sB7-H2 was linear positive correlation with LDH(Lactate dehydragenase)(r=0.4789),hs-CRP(High-sensitivity)(r=0.246),WBC(White Blood Cell) (r=0.4007) and LPS(lipase)(r=0.2357)(P＜0.05,respectively).By the cutoffof sB7-H2,the sensitivity and specificity to judge above moderate pancreatitis were 88.9％ and 83.3％,to judge SAP were 96％ and 96％.Conclusion sB7-H2 has important clinical value in judging the severity of AP at early time with high sensitivity and specificity,and is linear correlated to the clinical severity indexes..