In vitro evaluation of casein phosphopeptides-amorphous calcium phosphate combined with Duraphat on the remineralization of early enamel caries using quantitative light-induced fluorescence
WU Xin-er
LIU Jin
LI Shu-fang
LI Jiao-jiao
Abstract：Objective To evaluate the remineralization effect of casein phosphopeptide-amorphous calcium phosphate(CPP-ACP)combined with Duraphat on artificially induced early enamel caries using quantitative light-induced fluores-cence(QLF)technology and scanning electron microscopy(SEM).Methods Thirty-four extracted premolars from pa-tients who sought treatment at the Department of Stomatology,Youjiang Medical University for Nationalities Affiliated Hospital,from October 2020 to August 2021 were used to prepare early enamel caries models.Two extracted teeth were randomly selected for observation of the surface morphology of the enamel after demineralization,which was recorded as the blank control tooth.The remaining 32 extracted teeth were subjected to remineralization treatment,and were random-ly divided into four groups of eight teeth each;according to the treatment method,they were recorded as deionized water group,CPP-ACP group,Duraphat group,and combined group(CPP-ACP+Duraphat).The QLF images were used to compare the fluorescence loss percentage(△F),deminer-alized area(Area),and lesion volume(△Q)before and af-ter 21 days of remineralization treatment in each group,and the surface morphology of the enamel at 21 days after remineralization treatment was observed under SEM.Results Before remineralization treatment,there was no signifi-cant difference in △F,Area,or △Q among the four groups(P＞0.05).After 21 days of remineralization treatment,com-pared with the pretreatment,the absolute value of △F,△Q,and Area of the CPP-ACP group,deionized water group,Du-raphat group,and combined group decreased,and the differences were statistically significant(P＜0.05).Compared with the other three groups,the absolute value of △F,△Q,and Area of the combined group were the smallest,and the differ-ences were statistically significant(P＜0.05).Under SEM observation,it was found that all four groups showed reminer-alization compared with the blank control teeth,with the changes in the deionized water group being smaller,and the CPP-ACP group,the Duraphat group,and the combined group showing obvious remineralization.The combined group showed better remineralization effect.Conclusion Compared with using CPP-ACP or Duraphat alone,using them to-gether for early enamel caries model will have better remineralization effects.
Keywords：quantitative light-induced fluorescenceremineralizationcasein phosphopeptides-amorphous calcium phos-phateDuraphatearly enamel caries
Publication Date：2024-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 670-675 )