Sonodynamic therapy as an adjuvant treatment for periodontitis in rats
LUAN Xiao-min
ZHUANG De-shu
DAI Jia-yin
LU Hai-yan
BI Liang-jia
Abstract：Objective To evaluate the effect of HMME-mediated adjunctive sonodynamic therapy (SDT)on periodontitis in rats,in order to provide theoretical and experimental evidence for the clinical application of SDT.Methods Ligatures were placed on the subgingival regions of the first maxillary molar in 54 rats to induce the periodontitis models.Then the rats were randomly divided into three groups with 18 rats in each group.(1)The control group was treated by normal saline (NS) to wash the periodontal pocket;(2)the scaling and root planing (SRP)group received SRP and was washed with normal saline;(3)the SRP plus SDT group was treated by SRP and washed with normal saline,and then was treated through subcutaneous injection of HMME 1 mL(40 μg/mL).After 90 min of incubation in the dark,the ultrasonic treatment at the frequency of 1 MHz and the intensity of 3W/cm2 was given for 10 minutes.SDT was performed every other day.Six rats from each group were sacrificed at 6,10,or 14 days postoperatively and periodontal tissue samples were taken for histological examination and immunohistochemical analysis.Results SRP plus SDT group had reduced number of inflammatory cells.The expression of receptor activator of nuclear factor kappa-B ligand (RANKL)was weakly positive,and the number of positive Tartrate-resistant acid phosphatase (TRAP) was significantly reduced (P ＜0.05),compared to the SRP and the control group (P ＜ 0.05).Conclusion SDT is an effective adjunctive therapy for periodontitis in rats.
Keywords：periodontitissonodynamic therapyhematoporphyrin monomethyl etherscaling
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 91-94 )