Effect of arecoline on the permeability of Hacat cell epithelia model and the regulation of calcium ion
CHEN Rong
HOU Dong-lan
YIN Xiao-min
GAO Yi-jun
Abstract：Objective To study the effect of arecoline on the permeability of Hacat cell epithelia model in vitro and the adjustment of Ca2+ on it.Methods Establish the Hacat cell epithelia monolayer model in vitro by culturing Hacat cell.The models were divided into control group (group A),arecoline treatment group (group B) and "calcium+ arecoline" treatment group (group C).The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatment with arecoline and calcium.Results In group B,the Lucifer Yellow Papp of each subgroup was higher than that of group A (P ＜ 0.05) and the Lucifer Yellow Papp increased as the arecoline concentration and treatment time were increased (P ＜ 0.05).In group C,after pretreatment with calcium,the Lucifer Yellow Papp of each subgroup were lower than that of group B at corresponding concentration and time points (P ＜ 0.05);while the Papp of each subgroup was higher than that of group A (P ＜ 0.05).Besides,the Lucifer Yellow Papp increased as the arecoline concentration and treating time were increased (P ＜ 0.05).Conclusion The increase of permeability of oral epithelia model by arecoline may play an important role in the pathogenesis and development of oral submucous fibrosis.The pretreatment with calcium,to some extent,can weaken the effect of arecoline on the permeability of Hacat cell monolayer model.
Keywords：oral submucous fibrosisOSFarecolinecalcium ionpermeabilityHacat cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 732-735,739 )