Effects of sialidase gene deletion on Porphyromonas gingivalis biofilm formation under stressful environmental conditions
TONG Tong
LI Chen
XU Xiao-yu
YANG Xue
GUO Yan
Abstract：Objective To compare the biofilm formation of P.gingivalis W83 and its sialidase-deficient mutant strain (△PG0352) under stressful conditions.Methods P.gingivalis W83 and △PG0352 were cultured in trypticase soy broth (TSB)and supplemented with 5 μghnL hemin and 1 μg/mL menadione under stressful conditions,including temperature,oxidatie stress and pH values.Biofilms were formed on the 96-well plates for four days,and stained with crystal violet.Detect the biofilm formation with spectrophotometer.Results Both P.gingivalis W83 and △PG0352 strains could form biofilms in normal culture conditions.P.gingivalis W83 formed more biofilm,compared to △PG0352 (P ＜ 0.05).The biofilm formations of P.gingivalis W83 and △PG0352 decreased in 34℃ and 41 ℃ (P ＜ 0.05).As the concentration of H2O2 increased,biofilm formations of both strains decreased more and more seriously.The biofilm formations of △PG0352 were less than those of P.gingivalis W83,when the ultimate concentration of hydrogen peroxide was 0.1,0.25 and 0.5 mmol/L.Both P.gingivalis W83 and △PG0352 formed less biofilm in alkaline condition (pH =9),△PG0352 even less,and were unable to form biofilm in acidic condition (pH =5).Conclusion Sialidase gene deletion can affect P.gingivalis biofilm formation.Biofilms formations of both P.gingivalis W83 and △PG0352 will decrease under stressful conditions.
Keywords：Porphyromonas gingivalissialidasebiofilm
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 436-440 )