Biological function and molecular mechanism of has-miR-16 in tongue squamous cell carcinoma
LI Qi
GENG Ya-jun
GUO Jing
E Li-hua
XU Yi-han
JIN Wu-long
Abstract：Objective To study the role and molecular mechanism of has-miR-16 in the development of tongue squa mous cell carcinoma (TSCC).Methods The expression of has-miR-16 was detected in 36 cases of TSCC tissues and matched normal tissues by using Quantitative real time PCR.The expression of has-miR-16 in TSCC cells (CAL-27 and SCC-9) and the normal human oral mucosa fibroblast cells (hOMF) was also analyzed by Quantitative real time PCR.The cell proliferation and migration of CAL-27 and SCC-9 were analyzed by MTT and wound healing assays after being transfected with has-miR-16 mimics or mimics control.The target gene of has-miR-16 was predicted by bioinformatics and verified by dual luciferase reporter assay.Results The expression of has-miR-16 in TSCC tissues was significantly lower than matched normal tissues (P ＜ 0.05).The expression of has-miR-16 was also down-regulated in CAL-27 and SCC-9 cells compared with hOMF cells (P ＜ 0.05).Over-expression of has-miR-16 inhibited cell proliferation and migration in CAL-27 and SCC-9 cells (P ＜ 0.05).MYB (Myeloblastosis oncogene) was the target gene of has-miR-16 in TSCC.Conclusion Has-miR-16 is down-regulated in TSCC tissue and cell lines;over-expression of has-miR-16 inhibites CAL-27 and SCC-9 cells proliferation and migration;has-miR-16 acts as tumor suppressor in TSCC,which may play its role via regulating MYB.
Keywords：has-miR-16tongue squamous cell carcinomaTSCCsuppressormyeloblastosis oncogeneMYB
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 373-377 )