Effect of phenytoin on the proliferation of human periodontal ligament stem cells under existing lipopolysaccha-ride
QIU Cai-qing
SUN Chao
WANG Bao-yan
QIU Ying-na
SUI Guo-long
LIU Li-mei
ZAI Li-ying
Abstract：Objective To evaluate the effects of phenytoin(PHT)on proliferation of human periodontal ligament stem cells(hPDLSCs)under existing lipopolysaccharide(LPS). Methods MTT method was used to observe the proliferation of hPDLSCs cultured in vitro under the action of 20μg/mL PHT+100 ng/mL LPS,5μg/mL PHT+100 ng/mL LPS,20μg/mL PHT and 5μg/mL PHT,respectively. Results Both 20μg/mL and 5μg/mL PHT could enhance the proliferation of hPDLSCs,and furthermore the effect of 20μg/mL PHT was stronger. The enhancing effects of 20μg/mL and 5μg/mL PHT on the proliferation of hPDLSCs remained no change when 100 ng/mL LPS was added. Conclusion PHT at low concentration can enhance the proliferation of hPDLSCs;a small amount of LPS does not affect biological effect of PHT in enhancing the proliferation of hPDLSCs.
Keywords：phenytoinlipopolysaccharidehuman periodontal ligament stem cellscell proliferation
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 600-603 )