Effects of glucocorticoid on osteoprotegerin expression in periodontal ligament cell
CHEN Jin
LI Qiu-lan
LUO He-ping
LI Qin
Abstract：Objective To investigate the effect of glucocorticoids on periodontal ligament cell(PDLC)expressing osteo-protegerin. Methods Healthy premolars extracted for orthodontic reasons from a 12- year- old boy were obtained,and periodontal tissues were collected and cultured to obtain PDLCs. PDLCs were obtained by means of limited dilutions and were characterized as positive cloned cells. Dexamethasone(DEX)was added into culture media and their effects on PDLC proliferation and OPG secretion were observed. The OPG concentrations in cell culture supernatants were detected by sandwich ELISA. Living cell numbers were demonstrated by MTT result. Results DEX above 40 ng/mL decreased the opg and opg/mtt values(P<0.001)but had no effect on mtt values(P>0.05). Conclusion Glucocorticoids might increase periodontal bone resorption by inhibition of OPG production in periodontal ligament cells.
Keywords：periodontiumcell cultureglucocorticoids
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 535-537 )