Influence of nuclear factor-κB/inhibitoryκB pathway on intracellular adhesion molecule-1 expression induced by P.gingivalisin endothelial cells
ZHANG Dong-mei
CHANG Chun-rong
PAN Ya-ping
Abstract：Objective To investigate whether intracellular adhesion molecule-1(ICAM-1)could be mediated by nucle-ar factor-κB(NF-κB)pathway in endothelial cells treated by P. gingivalis W83. Methods As we know production of ICAM-1 in endothelial cells could be increased by infection with P.gingivalis. Then the in vitro models of vascular endo-thelial cell infected with P. gingivalis W83 was established. Expression of ICAM-1 was examined by Real-time PCR and Western blotting in endothelial cells pretreated with P. gingivalis W83 for 8 h with or without the NF-κB antagonist MG132 at concentration of 5,10,20μmol/L respectively for 15,30,60 min. SAS 8.12 software was used for statistical anal-ysis;analysis of variance was done to compare the means. Results ICAM-1 production in endothelial cells increased by infection with P.gingivalis,MG132 could abrogate the production of ICAM-1 mRNA at concentration of 10μmol/L for 30 min,and the production of ICAM-1 was abrogated by MG132 at concentration of 20μmol/L for 15 min;in addition, the abrogation was more obvious at 30 and 60 min. Conclusion The induction of ICAM-1 by infection with P. gingiva-lis might be mediated by the NF-κB pathway in endothelial cells.
Keywords：P. gingivalisNF-κBICAM-1
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 417-420 )