Expression changes of transforming growth factor-β1 in rat dental pulp after direct pulp capping with mineral trioxide aggregate
ZHANG Xiao-fang
YAO Ya-peng
KANG Hong-ying
DONG Pei
Abstract：Objective To observe the dynamic expression of transforming growth factor-β1(TGF-β1)in rat dental pulp after direct pulp capping with mineral trioxide aggregate(MTA)and to evaluate the role of TGF-β1 on pulp injury and re-pair. Methods This study was conducted from October 2012 to March 2013 in the Central Laboratory of School of Sto-matology,China Medical University. Twenty-eight female Wistar rats were included in this study. The first molars on one side of the maxilla and mandible were mechanically exposed and the exposed dental pulps were directly capped with MTA. The contralateral molars received no treatment and served as controls. The rats were sacrificed 1d,3d,5d,7d,14d, 21d and 28d after direct pulp capping. TGF-β1 expression in pulp tissue was detected with immunohistochemical stain-ing. Results No TGF-β1 expression was detected in the control group. After direct pulp capping with MTA,TGF-β1 ex-pression gradually increased and reached peak expression on 5d post-direct capping(P < 0.05). TGF-β1 expression gradually decreased afterwards and reached normal stain on 21d post-direct pulp capping. TGF-β 1 was mainly ex-pressed in neutrophils,odontoblasts cells,vascular endothelial cells and fibroblasts. Conclusion After direct pulp cap-ping with MTA,TGF-β1 expression increased at first and then decreased. Our results suggest that TGF-β1 expression is related to the process of dental pulp repair.
Keywords：transforming growth factor-β1mineral trioxide aggregatedirect pulp cappingrepair after injuryrat
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 404-407 )