Study on SOX1 and PAX1 gene methylation detection in cervical cancer screening
YANG Rui-dong
TIAN Feng-xia
XIA Qing-an
ZHANG Zhi-yong
Abstract：Objective To investigate the methylation levels of PAX1 and SOX1 genes in different degrees of cervical le-sions,and provide reference for cervical cancer screening plans.Methods A total of 133 patients with positive high-risk human papilloma virus(HPV)results admitted to Luanzhou City People's Hospital from September to December 2021 were selected,and the methylation levels of PAX1 and SOX1 genes in exfoliated cervical cells were detected by spe-cific multiple PCR fluorescin-probe method.To study its relationship with DNA quantitative test,HPV typing test,thin-prep cytologic test(TCT)and histopathology results.Results The positive rate of methylation detection for high-grade cervical lesion and cervical cancer was 88.9％,which was significantly higher than that of DNA ploidy analysis(44.4％).The positive rate of methylation test was 33(50.0％)＞16(32.7％)＞58(30.8％)＞18(11.1％)＞52(10.5％).In comparison with the results of methylation and TCT,the positive rate of normal cervical tissue group(13.3％)was lower than TCT.In the comparison of diagnostic performance,methylation detection showed higher specificity(84.35％),positive predic-tive value(47.06％),and accuracy(84.96％)compared to the other two methods.Conclusion Methylation detection of SOX1 and PAX1 genes can be used as an auxiliary detection method for primary screening of cervical cancer,accurate-ly diverting precancerous lesions of cervical cancer,and providing diagnostic basis for early screening.
Keywords：cervical cancerSOX1PAX1DNA methylationhuman papilloma virus
Publication Date：2023-12-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1245-1248 )