Diversity analysis of the vaginal microbiota before and after cervical conization in women with HSIL
KANG Ling
MA Xiao
CAI Hui-hua
LV Li-yi
CHEN Mei-zhuang
ZHOU Hong-wei
LIU Mu-biao
Abstract：Objective To explore the diversity of the vaginal flora before and after cervical conization in patients with high-grade squamous intraepithelial lesion (HSIL).Methods Swabs were collected from December 2015 to August 2016 in Guangdong General Hospital and Zhujiang Hospital from 20 cases of HSIL patients before cervical conization and three months after cervical conization.Microbiota composition,alpha diversity and beta diversity among two groups were determined by analyzing the 16S rRNA V4 hypervariable region amplified from total genomic DNA prepared from the swabs.Results L.iners,Gardnerella,Prevotella and Atopobium was the predominant species in HSIL patients before cervical conization,and the content of L.crispatus decreased significantly.While after cervical conization,content of L.iners,Gardnerella,Prevotella and Atopobium decreased,while content of L.crispatus increased.Conclusion High abundant of L.iners,Gardnerella,Prevotella and Atopobium and low abundant of L.crispatus maybe was the risk factors of HR-HPV infection.
Keywords：high-grade squamous intraepithelial lesionvaginal floraBIPES16S rRNA
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 949-954 )