The study of genomic amplification of the human telomerase gene in cytological specimens by FISH for the diagnosis of cervical dysplasia and cervical cacinoma
YIN Ge-ping
LI Juan
ZHU Tong-yu
HUANG Hai-shi
ZHAO Xiao-li
ZHAO Xiu-e
HE Jun-mei
Abstract：Objective To investigate the genomic amplification of the human telomerase gene(hTERC)in cytogic specimen by fluorescence in situ hybridization(FISH)for the diagnosis of cervical dysplasia and cervical cacinoma.Mathods One hundred and senven cases of cervical cytology specimens in pap-smears.20 cares with negtive for intraepithelial lesion or malignancy(NILM)for control group,87 cases of study group including 8 with CIN Ⅰ stage,19 with CIN Ⅱ/Ⅲ,7 with cacinoma in situ,14 with invasive-type of squamous cacinoma in Ⅰ stage,24 with≥Ⅱ stage and 15 followedup cares after treatment by LEEP(Loop Electrosurgical Excision Procedure)(12 cases)and radical surgery(3 cares)were detected their hTERC genomic amplification by dual-color interphase FISH,and compared with cytologic(TCT)and histologic results.The pathologic result is the fainal diagnosis.Results(1)The diagnostic standard from the control group was≤6.0%.＞6.0%meaned positive.(2)The genomic amplification rates of hTERC and positive percent rates were found 9.17±4.62 and 50.0%(4/8)in CIN Ⅰ;16.00±14.44 and 84.2%(16/19)in CIN Ⅱ～Ⅲ;19.67±13.26 and 85.7%(6/7)in cacinoma in situ.There was statistically significant difference between in the control and study groups(P＜0.01),and among in the CIN Ⅰ,CIN Ⅱ～Ⅲ,cacinoma in situ,Ⅰ stage and in≥Ⅱ stage groups(P＜0.05).Conclusion The investigation of the genomic amplification of hTERC is a better marker in present to screen cevical cytological specimens for CIN,espicially in differentiating its maligancy.
Keywords：fluorescence in situ hybridizationcervical intraepithelial neoplasiacervical cacinomatelomerase
Publication Date：2010-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 201-204 )