Exogenous PUMA sensitizes HPV positive cervical cancer cells to irradiation in vitro
CHEN Yan
QIAN Hai-li
YANG Juan-jun
LIN Chen
XIANG Yang
Abstract：Objective To investigate whether exogenous PUMA (p53 up-regulated modulator of apoptosis) suppresses the growth of HPV positive cervical cancer and sensitizes HPV positive cervical cancer cells to irradiation. Methods An adenovirus expressing PUMA (Ad-PUMA), alone or in combination with irradiation, was used to treat HPV positive cervical cancer cells HeLa and CaSki. The growth inhibitory and pro-apoptotic effects of PUMA in vitro were examined. The efficacy of PUMA and p53 in suppressing the growth of HeLa and CaSkj were also compared. The expression of PUMA was examined in HeLa cell treated with irradiation. Results (1) Ad-PUMA suppressed the growth of HPV positive cervical cancer cells, and was more efficient than Ad-p53. (2) Irradiation induced PUMA expression in HeLa cell. (3)Combination of Ad-PUMA and irradiation significantly suppressed the cell growth, and increased the percentage of apoptotic cells than separate treatment. Conclusion Our data indicate that transfection of cervical cancer cells with Ad-PUMA is a potential novel approach to the therapy of HPV positive cervical cancer.
Keywords：PUMAcervical cancergene therapyapoptosis
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 598-601 )