Coagulation factor Ⅻ facilitate EOC cells invasion potential via stimulating 1L-8 secretion from monocytes
WANG Rui-li
ZHANG Ting
MA Zheng-wen
WANG Ying
DI Wen
XU Hong
MA Qing-liang
CHENG Zhang-ping
WANG Xi-peng
Abstract：Objective To explore the impact of monocytes (MOs) stimulated by F Ⅻ on the invasion potential of EOC. Methods Monocytes isolated from female healthy donors were culture and stimulated with Factor Ⅻ for 12 h. Tumor associated macrophage (TAM) were derived from ascites of EOC. India ink engulfing test was used to detect phagocytic capacity of MOs stimulated by F Ⅻ. Invasive potential of EOC, including ES-2, SKOV-3, HO-8910 cells were investigated by Matrigel invasion assay. The signaling pathway involved in MOs activated by F Ⅻ were measured by Transfactor family Kits, including AP-1 ,STAT, Inflammation 1 and Oncogene 3. Results (1) The percentage of engulfing Indian ink were 0.66 s0.04 in TAM and O. 36 s0.03 in monoeytes stimulated by FⅫ, which were siguificanfly higher than that mono-cytes from healthy donors(0.27±0.018) (P < 0.01 and P = 0.033). (2) ES-2 cells were shown more invasive cell number when the supematant from MOs+ Ⅻ was used as chemo-attractant (110.9±5.046), fewer than that of ascites group (157.5±14.86), but significantly more than that of negative control group in which cells were cultured with usualmedium (39.14±10. 91). When adding Centuximab into supernatants, decreased invasive cell numbers (42.43±9.097) were observed. Conclusion FⅫ might educate MOs differentiation toward TAM like cells, thus accelerated me-tastasis and invasion of EOC in the peritoneum.
Keywords：ovarian cancermonocytecoagulation factor Ⅻtumor associated macrophageIL-8
Publication Date：2009-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 594-597 )