AStudy on the microdissection method for isolation of human ovarian follicles
Abstract：Objective To establish a method for the isolation and collection of human ovarian follicles. Methods Four isolation methods,including mechanical pipetting, collagenase digestion, microdissection and the combination of collagenase digestion and microdissection, were performed and compared. The quantity of follicles from various phases of age was observed. Results The quantity of follicles decreased with the increasing age. In comparison with mechanical pipetting and collagenase digestion, more follicles( P＜ 0.01 ) and various follicles, including primordial, primary and secondary follicles,were obtained by using the method of collagenase digestion combined with microdissection despite its time consuming( P ＜0.01 ). Follicles in each group were selected randomly and cultured in vitro. After 48h, follicles maintained normal morphology under inverted microscope and produced detectable E2. Conclusion Collagenase digestion combined with microdissection is an efficient method for isolation and collection of follicles from human ovaries.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 222-224 )