Experimental study on mitomycin C resistance and its reversion in human cervical cancer
Abstract：Objective To determine the reversion of mitomycin(MMC) resistance by SDZ PSC833 and verapamil (VER) in human cervical cancer in vitro. Methods The reversion of mitomycin resistance by SDZ PSC833 or VER was detected for human cervical cancer cell ( Hela ) and its resistant subline Hela/MMC in vitro.. Results Both nontoxic doses of SDZ PSC833 and VER (1～3mg/L) might enhance the cytotoxicity of MMC in Hela and Hela/MMC and could result in almost complete or partial reversion of MMC -resistance of Hela/MMC. 3H -TdR incorporation test indicated that SDZ PSC833 or VER enhanced the inhibition of DNA synthesis in Hela and Hela/MMC cell. Moreover, the effect of SDZ PSC833 on reversing drug resistance was better than VER in vitro. Conclusions SDZ PSC833 and VER can overcome mitomycin resistance of Hela/MMC in vitro. SDZ PSC833 will be a better candidate for reversing multidrug resistance than verapamil in clinic.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 142-144 )