Effect of digoxin on the invasion ability of non-Hodgkin lymphoma raji cells and its mechanism

doi:10.19538/j.ek2018020614

Abstract：Objective To study the effect of digoxin on the invasion ability of non-hodgkin lymphoma (NHL) raji cells,and to preliminarily investigate its mechanism of action.Methods The 30 tissue samplec of non-Hodgkin lymphoma were taken in the Pathological Department of the Fourth Hospital of Hebei Medical University from Oct.2010 to Dec.2013.(1) The protein expression of Tiam-1 and Rac-1 in N HL were detected by i mmunohistochemical S-P method.(2)With different concentration of digoxin,the changes in expression levels of Tiam-1 and Rac-1 within raji cells were detected via cell invasion test and qRT-PCR.Results (1)Immunohistochemical result indicated:the positive rates of protein Tiam-1 (70％) and Rac-1 (73.33％) in NHL were higher than those in normal lymph node tissues with statistical significance (P ＜ 0.05).Correlation analysis indicated there was correlation between Tiam-1 and Rac-1,with a correlation coefficient of r =0.757 (P ＜ 0.01).(2)Cell invasion test indicated:after 48 h treatment by digoxin of different concentrations,the invasion rate reduced with the increasing digoxin concentration,and the results were statistically significant (P ＜ 0.01).(3) qRT-PCR result indicated:after 48 h treatment by digoxin of different concentrations,statistical difference was observed in the relative expression levels of Tiam-1 and Rac-1 between groups;the relative expression levels reduced with the increasing digoxin concentrations (P ＜ 0.05).Conclusion Digoxin,a cardiac glycoside,is able to inhibit lymphoma invasion or metastasis to some extent via regulating the expressions of Tiam-1 and Rac-1.