Expression differences of microRNA-126 in mast cell and its influence on release of IL-4
Abstract：Objective To observe the expression features of peripheral blood microRNA (miR)-126 in children with asthma and to investigate the influence of miR-126 downregulation on release of interleukin(IL)-4 in mast cells and its possible mechanism.Methods The peripheral blood of healthy subjects (control group) and astham children (asthma group) were enrolled.The asthma group included 6 children who were treated in Nanjing Children's Hospital from September to December of 2015.The levels of serum miR-126 were measured in both groups by quantitative real-time polymerase chain reaction (RT-PCR).After stimulation with lipopolysaccharide (LPS) on P815,miR-126 expression was detected by RT-PCR.P815 were transfected with miR-126 negative control (negative control group) and miR-126 inhibitor (miR-126 inhibitor group) respectively.The levels of IL-4 in supematants were detect by ELISA,and the levels of phosphorylated P38 were detected by Western Blot in two groups.Results The levels of peripheral blood miR-126 in asthma group were significantly higher than those in control group (P ＜ 0.05).In LPS stimulated P815,miR-126 expression increased (P ＜ 0.05).The levels of IL-4 and phosphorylated P38 in the miR-126 inhibitor group were lower than those in the negative control group,with statistical significance (P ＜ 0.05).Conclusion The expression of peripheral blood miR-126 increases in asthma children.Downregulation of miR-126 may suppress IL-4 release from mast cells by reducing the expression of phosphorylated P38.
Keywords：miR-126asthmachildmast cellIL-4
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 947-950 )