Relationship between gene polymorphism of β1 adrenergic receptor and outflow tract ventricular premature

doi:10.19538/j.ek20170806012

Abstract：Objective To investigate the relationship between β1 adrenergic receptor(β1-AR)gene polymorphism and outflow tract ventricular premature(OTVP)in Han Chinese children.Methods A case-control study was performed.A total of 151 children with OTVP were recruited,who were diagnosed in Beijing Children's Hospital from January 2014 to December 2015,and 150 healthy children served as controls.Peripheral blood sanples were collected from all subjects and the genotype of β1-AR were determined by PCR,followed by direct sequencing.The frequency distribution of genotypes was compared between the OTVP and control groups.The association between polymorphism of β1-AR and OTVP was analyzed.Results The frequencies of β1-AR genotypes Arg/Arg,Arg/Gly and Gly/Gly were 50.5％,38.5％ and 11％ in all children.The allelic frequencies of Arg389 and Gly389 were 69.8％ and 30.2％.The allelic frequencies of Arg389 in female chidren were higher than in male (P =0.005).There were significant differences in the genotype and allele frequencies of β1-AR gene Arg389Gly between the OTVP and controls (P=0.012,P =0.002),and Arg389 allele was a risk factor (OR =1.733,95％CI:1.218 ～2.466).No differences of genotype were observed between different genders in children with OTVP (P =0.088),but there were significant differences in the genotype between loads of ventricular premature and nonsustained ventricular tachycardia (P =0.029,P =0.019).Conclusion The gene polymorphism of Arg389Gly in β1-AR might be associated with the OTVP in Han Chinese children and Arg389 allele gene is a risk factor.The genotype of Arg/Arg might be a genetic susceptibility factor of high-level loads of ventricular premature and nonsustained ventricular tachycardia in children with OTVP.