Expression profile of long non-coding RNA in Hirschsprung's disease

doi:10.19538/j.ek2017060614

Abstract：Objective To investigate the long non-coding RNAs (lncRNAs) expression profiles in the bowels of patients with Hirschsprung disease (HSCR),and to explore the role of dysregulated lncRNAs in the pathogenesis of HSCR.Methods Thirty pairs of tissue samples,including aganglionic segments and ganglionic segments,were obtained from patients with HSCR during operation.Then lncRNA microarrays were used to investigate the expression profiles in 3 pairs of specimens coupled as HSCR tissues compared with matched normal colon tissues.Candidate genes were selected from these differentially expressed lncRNAs based on artificial criterion (raw signal intensity≥ 100;fold change≥ 3) and then validated by quantitative real-time polymerase chain reaction (qRT-PCR) in 27 pairs of tissues.Results A total of 217 lncRNAs in aganglionic colon segment were found to be more than two fold greater than that in ganglionic segment tissues,including 13 up-regulated and 204 down-regulated (P＜ 0.05).The expression of two lncRNAs were significantly changed in aganglionic tissues compared with the ganglionic tissues after qRT-PCR validation (P ＜ 0.05),whereas the change of other lncRNAs selected from artificial criterion were without statistical difference (P＞0.05).Conclusion Abnormal expression of lncRNAs is found in HSCR aganglionic segments,suggesting that lncRNAs may be involved in the pathogenesis of HSCR.