A study on platelet indices and platelet membrane glycoprotein in children with acute lymphocytic leukemia

doi:10.19538/j.ek2017050612

Abstract：Objective To investigate the change of the platelet state and function in acute lymphocytic leukemia.Methods The changes of platelet indices (PLT,PCT,MPV,PDW),immature platelet fraction (IPF％),immature platelet counts (IPC),granule membrane glycoprotein of platelet (CD62p) and PAC-1 were obtained by using automatic blood cell analyzer and whole blood flow cytometry (FCM) respectively,in children with acute lymphocytic leukemia (ALL),ALL in first complete remission (ALL-CR1) and children undergoing elective surgical procedure (control group).Results (1)Without addition of platelet agonists ADP,expression of platelet surface activated CD62p and PAC-1 in ALL was higher than that in control group (P ＜ 0.05),while expression of platelet surface activated PAC-1 in ALL-CR1 was higher than that in control group (P ＜ 0.05),and lower than that in ALL (P ＜ 0.05);(2)With addition of platelet agonists ADP,expression of platelet surface activated CD62p and PAC-1 in ALL was lower than that in control group (P ＜ 0.05),while expression of platelet surface activated PAC-1 in ALL-CR1 was lower than that in control group (P ＜ 0.05),and higher than that in ALL (P ＜ 0.05);(3) PLT,PCT and MPV in ALL was lower than that in control group and ALL-CR1 (P＜0.05);(4) IPF％ in ALL was higher than that in control group and ALL-CR1 (P ＜ 0.05),and IPC was lower than that in control group and ALL-CR 1 (P ＜ 0.05).Conclusion The children who are newly diagnosed with acute lymphocytic leukemia have peripheral platelet reactivity and platelet function defects.Platelet indices and platelet membrane glycoprotein can be used as effective indicators to judge the effect on ALL.