Association between polymorphisms of monocyte chemoattractant protein 1 gene and Kawasaki disease

LI Xiao-qing; ZHOU Nan; CAO San-cheng; YANG Wei-na; ZHU Xie-ying

Abstract：Objective To investigate the polymorphisms of monocyte chemoattractant protein 1 (MCP-1) gene in chil-dren with Kawasaki disease (KD) in Shannxi province and association between the polymorphisms and coronary artery lesion(CAL) in KD. Methods A total of 2 in-patients who were diagnosed with KD were divided into two groups: 39 cases with CAL group (CAL group) and 23 cases without CAL group(NCAL group); in addition, 60 healthy children were randomly regarded as control group, respectively. The -2518 codon genotype of MCP-1 gene were screened with polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) , and the serum concentration of MCP-1 were detected by enzyme linked immunosorbent assay (ELISA) for all of them. Results 1. The allele frequen-cy of the MCP-1 gene regulatory region -2518 G was significantly higher in heathy children of Shannxi region(53.3%) than in Caucasian and African (29% vs 22%), while it had no statistic significance compared with Korean and Mexican (65% vs 47%). 2. The allele frequency of MCP-1 gene mutation had significant difference between KD patients and heathy children in Shannxi region. 3. The serum MCP-1 concentration of patients with G/G genotype was remarkably in-creased compared with that of patients with C/A or A/A genotype in KD group (P < 0.01). 4. The level of MCP-1 was significantly higher in CAL group than in NCAL group. Compared with the serum MCP-1 concentration of patients with G/G or G/A genotype in NCAL group, there was significant increase in CAL group (P < 0.05). Conclusion There is polymorphic difference of allele frequency of-2518 co-don G/A mutation of MCP-1 gene between children in Shannxi region and different ethnic populations, while polymorphisms of the gene may not contribute to sensitivi-ty for KD. Nevertheless, the polymorphic deviation may influence KD accompanied by CAL to some extent.