A study on protective effect of magnesium on hypoxic-ischemic brain damage

doi:10.3969/j.issn.1005-2224.2001.03.010

Abstract：Objective To investigate the neuroprotective effect of magnesium sulfate on hypoxic-ischemic brain damage and its relation with apoptosis. Methods The HIBD model was produced using 7-day old Wistar rats subjected to unilateral common carotid artery ligation followed by hypoxic state(8% oxygen +92% nitrogen at 37℃) for 2 hours. The rats were divived into three groups:control group、HI group and MgSO4-treated group. The rats were sacrificed 72 hours after hypoxic ischemia. Hematoxylin-Eosin(HE) staining and terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) were used to evaluate the histopathological features and the number of apoptic cells in brain tissue of the three groups. Results It was found that the numbers of apoptic cell were markedly increased in hypoxic-ischemic brain tissue(t=-56.61, P＜0.001) and decreased significantly in MgSO4-treated group compared with that in HI group(t=10.39,P＜0.001). The neuronal degenerations in cortex were reduced in MgSO4-treated group. Conclusion The data indicate that magnesium has protective effect on HIBD,which has relation with inhibiting apoptosis.