Pridiction of plasma differentially expressed miRNAs for postoperative recurrence of meningiomas

WANG Xiao-wei; GAO Xue-jun; ZHANG Bo; ZHENG An-xi; LUO Shuang

doi:10.13798/j.issn.1009-153X.2024.07.005

Abstract：Objective To explore the role of differentially expressed microRNAs (miRNAs) in plasma in predicting postoperative recurrence of meningiomas. Methods Plasma samples of 5 patients with meningiomas and 5 healthy subjects were prospectively collected from May to September 2017. TaqMan Low-Density Array (TLDA) analysis was used to identify differentially expressed miRNAs. Ninty-seven patients with meningiomas were prospectively collected from November 2017 to November 2023. Preoperative PCR was used to detect differentially expressed miRNAs in plasma,and tumor recurrence was determined by MRI at the last follow-up. Results A total of 9 differentially expressed miRNAs (miR-21,miR-219-5p,miR-375,miR-190a,miR-451,miR-224,miR-107,miR-539,miR-197) were identified by TLDA analysis. Compared with patients with non-recurrent meningiomas,the expression levels of miR-21,miR-219-5p,miR-375,and miR-190a in the plasma of patients with recurrent meningiomas were significantly increased (P<0.05),while the expression levels of miR-451,miR-224,miR-107,miR-539,and miR-197 in the plasma were significantly decreased (P<0.05). At the last follow-up,15 patients (15.46％) had recurrence. Multivariate Cox regression analysis indicated that,high levels of miR-21,miR-219-5p,and miR-190a in plasma,and low levels of miR-107,miR-539,and miR-224 in plasma were closely related to postoperative recurrence of meningiomas (P<0.05). Kaplan-Meier curve analysis showed that patients with plasma miR-21≥2.64,miR-219-5p ≥2.72,miR-190a ≥1.82,and plasma miR-107 ≤3.78,miR-539 ≤4.69,and miR-224 ≤4.59 had significantly shorter recurrence-free survival time (P<0.05). Conclusion Patients with meningiomas have differentially expressed plasma miR-21,miR-219-5p,miR-375,miR-190a,miR-451,miR-107,miR-539,miR-224,miR-197,and miR-190a,and monitoring these miRNAs can assist in predicting the risk of postoperative recurrence of meningiomas.