Effect of colchicine on the sensitivity of glioma cells to temozolomide chemotherapy
WU Yu
ZHU Xin-yi
JIANG Hong-xiang
CHEN Qian-xue
Abstract：Objective To investigate the effect of colchicine on the sensitivity of glioma cells to temozolomide(TMZ)chemotherapy and its mechanism.Methods Different concentrations of TMZ were used to treat human glioma cell lines U87 and A172,and to construct TMZ-resistant cell lines U87/TR and A172/TR.After colchicine(10,30 ng/ml)treatment for 1,2,and 3 days,cell viability,cell proliferation,and cell cycle were detected by cell counting,BrdU method,and flow cytometry,respectively.The differentially expressed microRNAs(miRNAs)in glioma tissues were analyzed by bioinformatics methods,and their target genes were predicted.Then,qRT-PCR was used to detect the expression of these miRNAs in U87/TR and A172/TR cells,and the expression of miRNAs was regulated to observe the changes of colchicine effects.Results Colchicine significantly inhibited the proliferation of U87/TR and A172/TR cells(P<0.05),with time-and dose-dependent manners(P<0.05).Colchicine significantly blocked mitosis of U87/TR and A172/TR cells,and induced cell cycle arrest in the G0/G1 Phase.Colchicine at the concentration of 30 ng/ml significantly increased the inhibitory effect of TMZ on proliferation of U87/TR and A172/TR cells(P<0.05),and significantly reduced the IC50 value of TMZ(P<0.05).Bioinformatics analysis showed that miR-330-3p,miR-491-5p,miR-6782-5p,miR-31-5p,miR-330-5p,miR-137,and miR-433-3p were differentially expressed in glioma tissues.Colchicine significantly upregulated the expression of miR-330-3p in U87/TR and A172/TR cells(P<0.05),and significantly inhibited the expression of miR-330-3p target gene ErbB(P<0.05).Inhibition of miR-330-3p significantly reversed the biological effects of colchicine(P<0.05).Conclusions Colchicine can inhibit the proliferation of TMZ-resistant glioma cells,and the mechanism may be involved in the regulation of miR-330-3p/ErbB signaling pathway.
Keywords：GliomaU87 cellsA172 cellsTemozolomideColchicineChemotherapy sensitivity
Publication Date：2024-02-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 97-104 )